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CelLytic TM MEM 蛋白提取试剂盒
CelLyticTM MEM Protein Extraction Kit
CelLytic TM MEM 蛋白提取试剂盒
CelLyticTM MEM Protein Extraction Kit
基本信息
NACRES | NA.56 |
General description【一般描述】 | 膜蛋白占据了生物体基因组大约20-30%的成分,充当着胞内的看门人、调节器和感应器的角色。它们具有多样的细胞功能,例如将细胞与外部的毒素隔离开来,作为胞内信号级联的起始点,以及维持临界离子浓度。 CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines. |
Application【应用】 | CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting. |
Legal Information【法律信息】 | CelLytic is a trademark of sigma-aldrich Co. LLC |
Components【组分】 | 组份不可单独销售 Lysis and Separation Buffer 50 mL Wash Buffer for CelLytic MEM 50 mL Sodium Chloride, 4M Solution 1.5 mL |
产品性质
Quality Level【质量水平】 | 200 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
suitability【适用性】 | 提供足够进行80次检测的试剂。 |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |
Flash Point(F)【闪点(F)】 | 188.6 °F - closed cup |
Flash Point(C)【闪点(C)】 | 87 ℃ - closed cup |