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SYBR® Green Extract-N-AmpTM植物PCR试剂盒

SYBR® Green Extract-N-AmpTM Plant PCR Kit
产品编号:3246200
产品类别:试剂盒
纯度规格:sufficient for 100 preparations
包装规格:1 KIT
市场价:4170元
优惠价:立即咨询

SYBR® Green Extract-N-AmpTM植物PCR试剂盒

SYBR® Green Extract-N-AmpTM Plant PCR Kit

基本信息
NACRES
NA.55
General description【一般描述】
SYBR® Green Extract-N-Amp Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
Features and Benefits【特点和优势】
  • Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • No loading buffers or tracking dyes required for gel analysis
  • SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
  • SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
  • Compatible with high-throughput requirements for genetic analysis of plants
  • No long enzymatic digestions
  • Extract stable at 4℃ for at least 6 months
Other Notes【其他说明】
该实验方案采用20 μl规格的PCR反应。
Legal Information【法律信息】
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
产品性质
Quality Level【质量水平】
200
usage【用途】
sufficient for 100 preparations
sufficient for 100 reactions
feature【特点】
dNTPs included
hotstart
technique(s)
qPCR: suitable
Detection Method
SYBR® Green
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
安全信息
Pictograms【象形图】
GHS07,GHS09
Signal word【警示用语:】
Warning
Hazard Statements
H315 - H317 - H319 - H411
Precautionary Statements
P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
Hazard Classifications【危险分类】
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
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