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脂解(脂肪细胞)检测试剂盒

Lipolysis (Adipocyte) Kit
产品编号:3105639
产品类别:试剂盒
纯度规格:Sufficient for 5 g of tissue
包装规格:1 KIT
市场价:8200元
优惠价:立即咨询

脂解(脂肪细胞)检测试剂盒

Lipolysis (Adipocyte) Kit

基本信息
EC number【EC 号】
259-322-7
NACRES
NA.25
General description【一般描述】
Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.
Principle【原理】
The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λex = 535/ λem = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present.
Components【组分】
组份不可单独销售
Collagenase (0.2%)
Collagenase Stop Buffer
Adipocyte Wash Buffer
Adipocyte Lipolysis Buffer
Glycerol Assay Buffer
Glycerol Probe, in DMSO
Glycerol Enzyme Mix
Glycerol Standard, 100 mM
Isoproterenol, 10 mM
Cell Strainer
产品性质
Detection Method
colorimetric
fluorometric
storage temp.【储存温度】
−20℃
suitability【适用性】
该试剂盒适用于从组织中分离脂肪细胞和脂解作用的测定。
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H315 - H319 - H335
Precautionary Statements
P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
Hazard Classifications【危险分类】
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs【靶器官】
Respiratory system
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
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