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脂解(脂肪细胞)检测试剂盒
Lipolysis (Adipocyte) Kit
脂解(脂肪细胞)检测试剂盒
Lipolysis (Adipocyte) Kit
基本信息
EC number【EC 号】 | 259-322-7 |
NACRES | NA.25 |
General description【一般描述】 | Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes. |
Principle【原理】 | The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λex = 535/ λem = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present. |
Components【组分】 | 组份不可单独销售 Collagenase (0.2%) Collagenase Stop Buffer Adipocyte Wash Buffer Adipocyte Lipolysis Buffer Glycerol Assay Buffer Glycerol Probe, in DMSO Glycerol Enzyme Mix Glycerol Standard, 100 mM Isoproterenol, 10 mM Cell Strainer |
产品性质
Detection Method | colorimetric fluorometric |
storage temp.【储存温度】 | −20℃ |
suitability【适用性】 | 该试剂盒适用于从组织中分离脂肪细胞和脂解作用的测定。 |
安全信息
Pictograms【象形图】 | GHS07 |
Signal word【警示用语:】 | Warning |
Hazard Statements | H315 - H319 - H335 |
Precautionary Statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
Hazard Classifications【危险分类】 | Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3 |
Target Organs【靶器官】 | Respiratory system |
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 3 |