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颗粒酶B活性检测试剂盒
Granzyme B Activity Assay Kit
颗粒酶B活性检测试剂盒
Granzyme B Activity Assay Kit
CTLA1
GZMB Activity Assay Kit
GZMB活性检测试剂盒
基本信息
EC number【EC 号】 | 200-664-3 |
NACRES | NA.84 |
General description【一般描述】 | GZMB codes for granzyme B. Granzyme B (gzm B) is a peptidase that induces cytotoxic T lymphocyte (CTL)-mediated DNA fragmentation and apoptosis in allogeneic target cells. It cleaves after aspartic acid residues in substrates. Granzyme B-mediated release of mitochondrial cell death factors are required for the activation of caspase-3. Furthermore, the degradation of granzyme B is known to decrease the susceptibility of cancer cells to NK-cell-mediated lysis during hypoxia. Granzyme B activity assays can be used for studying T cell immunity, CTL responses, cell-mediated cytotoxicity, and apoptosis. Gzm B is a pro-apoptotic member of the granzyme family. It is a neutral serine protease with Aspase activity. Gzm B is expressed exclusively in the granules of activated cytolytic T cells, natural killer cells and lymphokine-activated killer cells. |
Principle【原理】 | Granzyme B activity is determined using an enzyme-catalyzed reaction in which the non-fluorescent substrate, Ac-IEPD-AFC, is hydrolyzed resulting in the release of AFC (λex = 380/λem = 500 nm) proportional to the enzymatic activity present. One unit of granzyme B is the amount of enzyme that hydrolyzes 1 pmole of the substrate per minute at 37 ℃. |
Components【组分】 | 组份不可单独销售 Granzyme B Assay Buffer Granzyme B Substrate Granzyme B Positive Control AFC Standard, 1 mM |
产品性质
usage【用途】 | sufficient for 100 fluorometric tests |
Detection Method | fluorometric |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... GZMB(3002) mouse ... GZMB(14939) rat ... GZMB(171528) |
suitability【适用性】 | 适于检测生物样本中的颗粒酶B活性和筛选颗粒酶B的抑制剂。 |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |