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TSKgel® Phenyl-5PW RP HPLC Column
| 产品编号： | 4170211 |
| 规格： | phase phenyl, L × I.D. 7.5 cm × 2 mm, 10 μm particle size |
| 包装规格： | 1 EA |
| 产品类别： | 进口试剂 |
| 优惠价： | 立即咨询 |
产品别名
TSKgel® Phenyl-5PW RP HPLC Column
TSKgel Reversed Phase HPLC Column (Polymer-based)
基本信息
| eCl@ss | 32110501 |
| General description【一般描述】 | TSKgel Phenyl-5PW RP columns are filled with 10 micron porous polymethacrylate particles, the surface of which is covered with a high density of phenyl groups that causes proteins to be retained in a low salt buffer mobile phase and requiring a solvent gradient for their elution, as is the protocol in reversed phase chromatography. In contrast, TSKgel Phenyl-5PW has such a low density of phenyl groups on the surface that proteins can only be retained at high salt concentration and eluted by a decreasing salt gradient, which, of course, is the protocol used in hydrophobic interaction chromatography. TSKgel Phenyl-5PW RP columns can withstand rigorous cleaning with either acid or base. The 1000 A pore size accommodates globular protein samples up to 1,000,000 Da. Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
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| Physical form【外形】 | Shipped in 30% methanol - 70% water. |
| Recommended products【推荐产品】 | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| Legal Information【法律信息】 | TSKgel is a registered trademark of Tosoh Corporation |
产品性质
| material【物料】 | stainless steel hardware |
| product line【产品线】 | TSKgel® |
| manufacturer/tradename | Tosoh Phenyl-5PW, 18756 |
| extent of labeling【标记范围】 | 1 meq/mL ligand density (estimate) |
| parameter【参数】 | no limitation salt concentration 0-100% organic solvent 0.12 mL/min flow rate 10 bar max. pressure 4-60 ℃ temperature |
| technique(s) | HPLC: suitable |
| L × I.D.【长度 × 内径】 | 7.5 cm × 2 mm |
| matrix【基质】 | polymer particle platform |
| matrix active group【基质活性基团】 | phenyl phase |
| Particle size【粒径】 | 10 μm |
| pore size【孔径】 | 1000 Å |
| operating pH | 2 - 12 |
| separation technique【分离技术】 | reversed phase |
