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TSKgel® Ether-5PW HPLC Column

产品编号:4170117
规格:phase ether, L × I.D. 1 cm × 2 mm, 10 μm particle size, pkg of 3 ea
包装规格:3 EA
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41701173 EA7410
基本信息
eCl@ss
32110501
General description【一般描述】
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Guard Cartridge for 2mmID TSKgel Ether-5PW column, 3pk
Recommended products【推荐产品】
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information【法律信息】
TSKgel is a registered trademark of Tosoh Corporation
产品性质
material【物料】
stainless steel
product line【产品线】
TSKgel®
packaging【包装】
pkg of 3 ea
manufacturer/tradename
Tosoh 42156
parameter【参数】
≤4 M salt concentration
≤50% organic solvent
0.12 mL/min flow rate
10-50 ℃ temperature
technique(s)
HPLC: suitable
L × I.D.【长度 × 内径】
1 cm × 2 mm
matrix【基质】
polymer particle platform
matrix active group【基质活性基团】
ether phase
Particle size【粒径】
10 μm
pore size【孔径】
1000 Å
operating pH
2 - 12
separation technique【分离技术】
hydrophobic interaction (HIC)
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