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TSKgel® Chelate-5PW HPLC Column
产品编号: | 4170098 |
规格: | phase iminodiacetic acid, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size |
包装规格: | 1 EA |
产品类别: | 进口试剂 |
优惠价: | 立即咨询 |
产品别名
TSKgel® Chelate-5PW HPLC Column
TSKgel Chelate-5PW HPLC Column and Guardgel Kit
Chelate-5PW
基本信息
eCl@ss | 32110501 |
General description【一般描述】 | High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins. TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well. Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides. |
Recommended products【推荐产品】 | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
Legal Information【法律信息】 | TSKgel is a registered trademark of Tosoh Corporation |
产品性质
material【物料】 | stainless steel |
product line【产品线】 | TSKgel® |
manufacturer/tradename | Tosoh Chelate-5PW, 08645 |
extent of labeling【标记范围】 | 20 μmol/mL resin ligand density |
parameter【参数】 | ≤20% organic solvent ≤3 M salt concentration ≤45 ℃ temperature 1.2 mL/min flow rate 10 bar max. pressure |
technique(s) | HPLC: suitable |
L × I.D.【长度 × 内径】 | 7.5 cm × 7.5 mm |
matrix【基质】 | polymer particle platform |
matrix active group【基质活性基团】 | iminodiacetic acid phase |
Particle size【粒径】 | 10 μm |
pore size【孔径】 | 1000 Å |
operating pH | 2 - 12 |
compatibility【相容性】 | technique used for affinity HPLC |
separation technique【分离技术】 | affinity |