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TRAIL, Human, Recombinant, NSO cells
产品别名
TRAIL, Human, Recombinant, NSO cells
Apo2L; TNF-Related Apoptosis-Inducing Ligand
产品性质
Quality Level【质量水平】 | 100 |
recombinant【重组】 | expressed in NSO cells |
Assay【测定】 | ≥97% (SDS-PAGE) |
form【形式】 | lyophilized |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze |
impurities【杂质】 | ≤1 EU/μg Endotoxin (EU/μg TRAIL) |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | −70℃ |
packaging【包装】 | 10 μg in Plastic ampoule |
基本信息
General description【一般描述】 | Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers. |
Biochem/physiol Actions【生化/生理作用】 | ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4. |
Reconstitution【重悬】 | Reconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions. |
Other Notes【其他说明】 | Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343. Mariani, S.M., et al. 1997. J. Cell Biol.137, 221. Pan, G., et al. 1997. Science277, 815. Pan, G., et al. 1997. Science276, 111. Sheridan, J.P., et al. 1997. Science277, 818. Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |