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Taq DNA Polymerase, GMP Grade

产品编号:4169544
规格:≥13000 units/mg protein, >98% (SDS-PAGE), sufficient for ≤2,000 reactions, Difficult Templates/Specialty Enzymes PCR
包装规格:1,000 U
产品类别:进口试剂
品牌:Roche
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41695441,000 U8630
产品别名

Taq DNA Polymerase, GMP Grade

polymerase

primer extension

dna amplification

PCR

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
2.7.7.7 ( BRENDA | IUBMB )
General description【一般描述】
Taq DNA Polymerase, GMP Grade, is manufactured under GMP conditions. It also fulfills the high quality and documentation requirements of research and development laboratories in the pharmaceutical and biotechnology industries. Taq DNA Polymerase, GMP Grade, gives high lot-to-lot consistency. Recombinant Taq DNA Polymerase (from Thermus aquaticus, recombinant E. coli), GMP Grade, is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD. Taq DNA Polymerase also accepts modified deoxyribonucleoside triphosphates as substrates for highly efficient DNA labeling with radionucleotides, digoxigenin, fluorescein, or biotin.
Application【应用】
  • Taq DNA Polymerase, GMP Grade, can be applied for:
  • PCR
  • RT- PCR
  • Other primer-extension reactions, such as sequencing and labeling
Unit Definition【单位定义】
One unit Taq DNA Polymerase, GMP Grade is defined as the amount of enzyme that incorporates 20 nmol of total deoxyribonucleoside-triphosphates into acid precipitable DNA within 60 minutes at +65 ℃ under the assay conditions stated in the package insert.

Unit Assay: Incubation buffer for assay on activated DNA:
67 mM Tris/HCl; pH 8.3/25 ℃, 5 mM MgCl2, 10 mM mercaptoethanol, 0.2% polydocanol, 0.2 mg/ml gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP, pH 8.3 (25 ℃).

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi [α-32P]-dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at 65 ℃ for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl
Other Notes【其他说明】
仅用于生命科学研究。不可用于诊断。
Legal Information【法律信息】
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Components【组分】
组份不可单独销售
Taq DNA Polymerase, GMP Grade 5 U/μl
PCR Buffer with MgCl<sub>2</sub> 10x concentrated
产品性质
Quality Level【质量水平】
100
description【描述】
5 U/βl
Assay【测定】
>98% (SDS-PAGE)
usage【用途】
sufficient for ≤2,000 reactions
Specific Activity
≥13000 units/mg protein
feature【特点】
Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no
manufacturer/tradename
Roche
parameter【参数】
72 ℃ optimum reaction temp.
technique(s)
PCR: suitable
input
purified DNA
optimum pH【最佳pH】
~9 (20 ℃)
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃ (−15℃ to −25℃)
packaging【包装】
1 kit containing 2 components
安全信息
Hazard Statements
H412
Precautionary Statements
P273 - P501
Hazard Classifications【危险分类】
Aquatic Chronic 3
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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