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Taq DNA Polymerase, GMP Grade
产品编号: | 4169544 |
规格: | ≥13000 units/mg protein, >98% (SDS-PAGE), sufficient for ≤2,000 reactions, Difficult Templates/Specialty Enzymes PCR |
包装规格: | 1,000 U |
产品类别: | 进口试剂 |
品牌: | Roche |
优惠价: | 立即咨询 |
产品别名
Taq DNA Polymerase, GMP Grade
polymerase
primer extension
dna amplification
PCR
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 2.7.7.7 ( BRENDA | IUBMB ) |
General description【一般描述】 | Taq DNA Polymerase, GMP Grade, is manufactured under GMP conditions. It also fulfills the high quality and documentation requirements of research and development laboratories in the pharmaceutical and biotechnology industries. Taq DNA Polymerase, GMP Grade, gives high lot-to-lot consistency. Recombinant Taq DNA Polymerase (from Thermus aquaticus, recombinant E. coli), GMP Grade, is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD. Taq DNA Polymerase also accepts modified deoxyribonucleoside triphosphates as substrates for highly efficient DNA labeling with radionucleotides, digoxigenin, fluorescein, or biotin. |
Application【应用】 |
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Unit Definition【单位定义】 | One unit Taq DNA Polymerase, GMP Grade is defined as the amount of enzyme that incorporates 20 nmol of total deoxyribonucleoside-triphosphates into acid precipitable DNA within 60 minutes at +65 ℃ under the assay conditions stated in the package insert. Unit Assay: Incubation buffer for assay on activated DNA: 67 mM Tris/HCl; pH 8.3/25 ℃, 5 mM MgCl2, 10 mM mercaptoethanol, 0.2% polydocanol, 0.2 mg/ml gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP, pH 8.3 (25 ℃). Incubation procedure: M13mp9ss, M13 primer (17mer) and 1 μCi [α-32P]-dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at 65 ℃ for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation. Volume Activity: 5 U/μl |
Other Notes【其他说明】 | 仅用于生命科学研究。不可用于诊断。 |
Legal Information【法律信息】 | Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
Components【组分】 | 组份不可单独销售 Taq DNA Polymerase, GMP Grade 5 U/μl PCR Buffer with MgCl<sub>2</sub> 10x concentrated |
产品性质
Quality Level【质量水平】 | 100 |
description【描述】 | 5 U/βl |
Assay【测定】 | >98% (SDS-PAGE) |
usage【用途】 | sufficient for ≤2,000 reactions |
Specific Activity | ≥13000 units/mg protein |
feature【特点】 | Difficult Templates/Specialty Enzymes PCR dNTPs included: no hotstart: no |
manufacturer/tradename | Roche |
parameter【参数】 | 72 ℃ optimum reaction temp. |
technique(s) | PCR: suitable |
input | purified DNA |
optimum pH【最佳pH】 | ~9 (20 ℃) |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ (−15℃ to −25℃) |
packaging【包装】 | 1 kit containing 2 components |
安全信息
Hazard Statements | H412 |
Precautionary Statements | P273 - P501 |
Hazard Classifications【危险分类】 | Aquatic Chronic 3 |
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |