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MISSION® Lenti microRNA, Mouse

产品编号:4167007
规格:mmu-miR-7034-5p
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4167007mmu-miR-7034-5p7540
基本信息
NACRES
NA.51
General description【一般描述】
Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.
Other Notes【其他说明】
Based on miRBase V20 Mature ID
Recommended products【推荐产品】
Two negative controls are available: NCLMIR001 and NCLMIR002
Legal Information【法律信息】
MISSION is a registered trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
product line【产品线】
MISSION®
concentration【浓度】
≥1x106 VP/ml (via p24 assay)
mature sequence【成熟序列】
UCCGGGAGGGAUGGAUGUGCU
Sanger mature/minor accession no.【Sanger mature/minor 登记号】
MIMAT0027972
Sanger microRNA accession no.【Sanger microRNA登记号】
MI0022883
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
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