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MISSION® Lenti microRNA Inhibitor, Human
产品别名
MISSION® Lenti microRNA Inhibitor, Human
Tough Decoy
TuD
基本信息
NACRES | NA.51 |
General description【一般描述】 | Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.
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Other Notes【其他说明】 | Based on miRBase V19 Mature ID |
Legal Information【法律信息】 | MISSION is a registered trademark of sigma-aldrich Co. LLC |
产品性质
Quality Level【质量水平】 | 200 |
product line【产品线】 | MISSION® |
concentration【浓度】 | ≥1x106 VP/ml (via p24 assay) |
technique(s) | capture ELISA: 106 TU/mL using p24 (Volume 200 uL) |
mature sequence【成熟序列】 | CACUGUGGGUACAUGCU |
Sanger mature/minor accession no.【Sanger mature/minor 登记号】 | MIMAT0016869 |
Sanger microRNA accession no.【Sanger microRNA登记号】 | MI0015847 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |