HDL Receptor SR-BI Inhibitor, BLT-1-CAS 321673-30-7-Calbiochem

C₁₂H₂₃N₃S

241.40

MFCD00571007

A cell-permeable thiosemicarbazone copper chelator that potently blocks the HDL receptor SR-BI- (scavenger receptor, class B, type I) mediated lipid uptake (IC₅₀ = 21 and 57 nM against DiI and CE uptake, respectively, from DiI-HDL and CE-HDL using hamster ldlA-7 cells expressing murine SR-BI) in a reversible manner, while exerting little effect toward intracellular membrane trafficking or cytoskeletal organization. BLT-1 chelates Cu⁺² in a 2:1 stoichiometric ratio and a half molar equivalent of CuCl₂ is shown to fully prevent zebrafish embryos from BLT-1-induced development defects, while BLT-1 can likewise be used to chelate excess amount of copper (>4 µM) and rescue embryos from copper toxicity.

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Nieland, T.J.F., et al. 2008. Biochemistry47, 460.
Raldua, D., and Babin, P.J. 2007. Toxicol. Lett.175, 1.
Nieland, T.J.F., et al. 2002. Proc. Natl. Acad. Sci. USA99, 15422.
Peterson, R.T., et al. 2000. Proc. Natl. Acad. Sci. USA97, 12965.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

white

DMSO: 100 mg/mL

ambient

2-8℃

Packaged under inert gas

11 - Combustible Solids

WGK 3