Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli

100

expressed in E. coli

>97% (SDS-PAGE)

lyophilized

Calbiochem^®

OK to freeze

≤1.0 EU/μg Endotoxin (EU/μg GM-CSF)

ambient

−70℃

10 μg in Plastic ampoule

Recombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells.
Recombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that stimulates the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells. Target cells include macrophages, granulocytes, and eosinophils. GM-CSF is produced by many cell types, including endothelial cells, fibroblasts, and T-lymphocytes. It can be extracted from most tissues in concentrations exceeding that of serum levels. While many cells produce GM-CSF constitutively, levels of this lymphokine increase rapidly in response to endotoxin, phorbol esters, and certain antigens.

ED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3

Toxicity: Standard Handling (A)

Lyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.

Anaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
Watanabe, S., et al. 1996. J. Biol. Chem.271, 12681.
Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
Gough, N.M., et al. 1985. EMBO J. 4, 645.

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11 - Combustible Solids

WGK 1