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Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

产品编号:4156735
规格:Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli, hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures.
包装规格:50 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415673550 μL7430
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.2.1.103 ( BRENDA | IUBMB )
General description【一般描述】
Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Warning【警告】
Toxicity: Standard Handling (A)
Unit Definition【单位定义】
One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
Physical form【外形】
Sterile-filtered in 20 mM Tris-HCl, pH 7.5.
Reconstitution【重悬】
There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
Other Notes【其他说明】
Murata, T., et al. 2003. Eur. J. Biochem.270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.
Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.
Scudder, P., et al. 1983. Biochem. J.213, 485.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
recombinant【重组】
expressed in E. coli
form【形式】
liquid
Specific Activity
≥14 U/mL
≥140 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
packaging【包装】
50 μL in Plastic ampoule
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
nwg
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