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Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli
产品编号: | 4156735 |
规格: | Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli, hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. |
包装规格: | 50 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.2.1.103 ( BRENDA | IUBMB ) |
General description【一般描述】 | Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8. Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. |
Warning【警告】 | Toxicity: Standard Handling (A) |
Unit Definition【单位定义】 | One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8. |
Physical form【外形】 | Sterile-filtered in 20 mM Tris-HCl, pH 7.5. |
Reconstitution【重悬】 | There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above. |
Other Notes【其他说明】 | Murata, T., et al. 2003. Eur. J. Biochem.270, 3709. Scudder, P., et al. 1986. Eur. J. Biochem.157, 365. Scudder, P., et al. 1984. J. Biol. Chem.259, 6586. Scudder, P., et al. 1983. Biochem. J.213, 485. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
recombinant【重组】 | expressed in E. coli |
form【形式】 | liquid |
Specific Activity | ≥14 U/mL ≥140 units/mg protein |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 50 μL in Plastic ampoule |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |