Dkk-1, Rat, Recombinant

expressed in mouse NSO cells

100

≥95% (SDS-PAGE)

lyophilized

Calbiochem^®

OK to freeze

≤1.0 EU/μg protein Endotoxin

ambient

−20℃

10 μg in Glass bottle

Recombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.
Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

ND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.

Toxicity: Standard Handling (A)

Lyophilized from 0.2 µm filtered PBS, BSA.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 2