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ChIPAb+ Histone H3 (CT)-ChIP Validated Antibody and Primer Set
产品别名
ChIPAb+ Histone H3 (CT)-ChIP Validated Antibody and Primer Set
Histone H3.1t, H3t, histone 3, H3/g
基本信息
eCl@ss | 32160702 |
NACRES | NA.32 |
General description【一般描述】 | All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Histone H3 (C-Term) set includes the Histone H3 (C-Term) antibody, a Normal Rabbit Serum, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The Histone H3 (C-Term) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H3 (C-Term)-associated chromatin. |
Specificity【特异性】 | Broad species cross-reactivity expected due to sequence homology. Recognizes C-Terminal region of Histone H3, Mr 17 kDa |
Immunogen【免疫原】 | KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3. |
Application【应用】 | This ChIPAb+ Histone H3 (C-Term) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µL of either Normal Rabbit Serum,or 1 µL of Anti-Histone H3 (C-Term) and the Magna ChIP Please refer to the EZ-Magna ChIP Western Blot Analysis: Representative lot data. Acid extracted proteins from HeLa cells untreated (lane 1) or treated with sodium butyrate (lane 2) or colcemid (lane 3) and recombinant Histone H3 (lane 4) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Histone H3, CT, pan (1:50,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H3 (~17 kDa). (Figure 3). |
Quality【质量】 | Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µL of either Normal Rabbit Serum, or 1 µL of Anti-Histone H3 (C-Term) and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of histone H3 associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1). Please refer to the EZ-Magna ChIP |
Physical form【外形】 | Unpurified Anti-Histone H3 (C-Term) (rabbit polyclonal). One vial containing 25 µL of rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%. Store at -20°C. Normal Rabbit Serum. One vial containing 25 μL of antiserum containing 0.05% sodium azide. Store at -20°C. ChIP Primers, human β-globin. One vial containing 75 μL of 5 μM of each primer specific for the human β-globin promoter. Store at -20°C. FOR: AGG ACA GGT ACG GCT GTC ATC REV: TTT ATG CCC AGC CCT GGC TC |
Analysis Note【分析说明】 | Control Includes normal rabbit serum and primers specific for human β-globin. |
Legal Information【法律信息】 | MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
clone【克隆】 | polyclonal |
species reactivity | chicken, mouse, yeast, Saccharomyces cerevisiae, human, rat |
manufacturer/tradename | ChIPAb+ Upstate® |
technique(s) | ChIP: suitable dot blot: suitable immunocytochemistry: suitable inhibition assay: suitable (peptide) western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_002107.3 |
UniProt accession no.【UniProt登记号】 | Q16695 |
shipped in【运输】 | dry ice |
packaging【包装】 | 25 assays per set. Recommended use: 1 μL of antibody per chromatin immunoprecipitation (dependent upon biological context). |
产品说明
Target description【目标描述】 | Approx. 17 kDa |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |