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ChIPAb+ Histone H3 (CT)-ChIP Validated Antibody and Primer Set

产品编号:4155646
规格:serum, from rabbit
包装规格:25 ASSAYS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415564625 ASSAYS6850
产品别名

ChIPAb+ Histone H3 (CT)-ChIP Validated Antibody and Primer Set

Histone H3.1t, H3t, histone 3, H3/g

基本信息
eCl@ss
32160702
NACRES
NA.32
General description【一般描述】
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Histone H3 (C-Term) set includes the Histone H3 (C-Term) antibody, a Normal Rabbit Serum, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The Histone H3 (C-Term) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H3 (C-Term)-associated chromatin.
Specificity【特异性】
Broad species cross-reactivity expected due to sequence homology.
Recognizes C-Terminal region of Histone H3, Mr 17 kDa
Immunogen【免疫原】
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
Application【应用】
This ChIPAb+ Histone H3 (C-Term) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µL of either Normal Rabbit Serum,or 1 µL of Anti-Histone H3 (C-Term) and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of histone H3 associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin as a positive locus, and GAPDH promoter primers (22-004) as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Acid extracted proteins from HeLa cells untreated (lane 1) or treated with sodium butyrate (lane 2) or colcemid (lane 3) and recombinant Histone H3 (lane 4) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Histone H3, CT, pan (1:50,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Histone H3 (~17 kDa). (Figure 3).
Quality【质量】
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µL of either Normal Rabbit Serum, or 1 µL of Anti-Histone H3 (C-Term) and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of histone H3 associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Physical form【外形】
Unpurified
Anti-Histone H3 (C-Term) (rabbit polyclonal). One vial containing 25 µL of rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%. Store at -20°C.
Normal Rabbit Serum. One vial containing 25 μL of antiserum containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, human β-globin. One vial containing 75 μL of 5 μM of each primer specific for the human β-globin promoter. Store at -20°C.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC
Analysis Note【分析说明】
Control
Includes normal rabbit serum and primers specific for human β-globin.
Legal Information【法律信息】
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
serum
clone【克隆】
polyclonal
species reactivity
chicken, mouse, yeast, Saccharomyces cerevisiae, human, rat
manufacturer/tradename
ChIPAb+
Upstate®
technique(s)
ChIP: suitable
dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable
NCBI accession no.【NCBI登记号】
NM_002107.3
UniProt accession no.【UniProt登记号】
Q16695
shipped in【运输】
dry ice
packaging【包装】
25 assays per set. Recommended use: 1 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
产品说明
Target description【目标描述】
Approx. 17 kDa
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
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