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ChIPAb+ FOXA1 Antibody

产品编号:4155641
规格:from rabbit, purified by affinity chromatography
包装规格:25 ASSAYS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415564125 ASSAYS6850
产品别名

ChIPAb+ FOXA1 Antibody

Forkhead box protein A1, Hepatocyte nuclear factor 3-alpha, HNF-3A, Transcription factor 3A, TCF-3A

基本信息
eCl@ss
32160702
NACRES
NA.32
General description【一般描述】
HNF-3A, Hepatocyte nuclear factor 3-alpha (also called FOXA1) is member of the Forkhead family of winged-helix transcription factors. This protein family has around 50 known transcription factors and play an important role in the regulation of cell differentiation, organogenesis, and gene expression. High levels of expression of HNF-3A has been observed in tumors arising from both the prostate and breast. Here it is believed this protein interacts with the androgen receptor in prostate tumors and the estrogen receptor α in breast tumors. In the case of ERα, HNF-3A interacts with cis-regulatory regions in heterochromatin and enhances the interaction of ERα with chromatin.
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ FOXA1 set includes the FOXA1 antibody, a Normal Rabbit IgG, and control primers which amplify a 138 bp region of ChIP Primers, Mouse Hnf4α enhancer. The FOXA1 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of FOXA1-associated chromatin.
Specificity【特异性】
This antibody recognizes HNF-3A/FOXA1.
Immunogen【免疫原】
Recombinant protein corresponding to the entire protein of human FOXA1
Application【应用】
Research Sub Category
Transcription Factors
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µl of either normal rabbit IgG, or 2 µl Anti-FOXA1 (Part No.CS207368) and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of FoxA1 associated DNA fragments was verified by qPCR using ChIP Primers, Mouse Hnf4α enhancer as a positive locus, and mouse Hnf4α promoter as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blotting Analysis:
Representative lot data.
Human pancreas tissue lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-FOXA1 (0.5 µg/mL).
Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. (Figure 3)
Arrow indicates FOXA1 (~51 kDa).
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of NIH/3T3 and HeLa cells using Anti-HNF-3A/FOXA1 (Red). Actin filaments have been labeled with Alexa Fluor 488 Dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains nucleus. (Figure 4)
This ChIPAb+ FOXA1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Research Category
Epigenetics & Nuclear Function
Quality【质量】
Chromatin Immunoprecipitation:
Representative lot data
Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit IgG,or 2 µL Anti-FOXA1 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of FOXA1- associated DNA fragments was verified by qPCR using ChIP Primers, Mouse Hnf4α enhancer (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Physical form【外形】
Anti-FOXA1 (Rabbit Polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C.
Concentration: 0.35 mg/mL
Normal Rabbit IgG. One vial containing 125 µg Rabbit IgG in 125 µL storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75 μL of 5 μM of each primer specific for Mouse Hnf4α enhancer. Store at -20°C.
FOR: TTC CAG CTG CCT TTA TCT CCC TGT
REV: TCT CCA CAC ATG TCC AGC AGC CT
Affinity purified
Analysis Note【分析说明】
Control
Includes normal rabbit IgG and primers specific for Mouse Hnf4α enhancer.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
human, mouse
manufacturer/tradename
ChIPAb+
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NP_004487
UniProt accession no.【UniProt登记号】
P55317
shipped in【运输】
dry ice
Gene Information
human ... FOXA1(3169)
packaging【包装】
25 assays per set. Recommended use: 2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
产品说明
Target description【目标描述】
~51 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
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