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ChIPAb+ FOXA1 Antibody
产品编号: | 4155641 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 25 ASSAYS |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
ChIPAb+ FOXA1 Antibody
Forkhead box protein A1, Hepatocyte nuclear factor 3-alpha, HNF-3A, Transcription factor 3A, TCF-3A
基本信息
eCl@ss | 32160702 |
NACRES | NA.32 |
General description【一般描述】 | HNF-3A, Hepatocyte nuclear factor 3-alpha (also called FOXA1) is member of the Forkhead family of winged-helix transcription factors. This protein family has around 50 known transcription factors and play an important role in the regulation of cell differentiation, organogenesis, and gene expression. High levels of expression of HNF-3A has been observed in tumors arising from both the prostate and breast. Here it is believed this protein interacts with the androgen receptor in prostate tumors and the estrogen receptor α in breast tumors. In the case of ERα, HNF-3A interacts with cis-regulatory regions in heterochromatin and enhances the interaction of ERα with chromatin. All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ |
Specificity【特异性】 | This antibody recognizes HNF-3A/FOXA1. |
Immunogen【免疫原】 | Recombinant protein corresponding to the entire protein of human FOXA1 |
Application【应用】 | Research Sub Category Transcription Factors Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µl of either normal rabbit IgG, or 2 µl Anti-FOXA1 (Part No.CS207368) and the Magna ChIP Please refer to the EZ-Magna ChIP Western Blotting Analysis: Representative lot data. Human pancreas tissue lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-FOXA1 (0.5 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. (Figure 3) Arrow indicates FOXA1 (~51 kDa). Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3 and HeLa cells using Anti-HNF-3A/FOXA1 (Red). Actin filaments have been labeled with Alexa Fluor This ChIPAb+ FOXA1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. Research Category Epigenetics & Nuclear Function |
Quality【质量】 | Chromatin Immunoprecipitation: Representative lot data Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit IgG,or 2 µL Anti-FOXA1 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of FOXA1- associated DNA fragments was verified by qPCR using ChIP Primers, Mouse Hnf4α enhancer (Figure 1). Please refer to the EZ-Magna ChIP |
Physical form【外形】 | Anti-FOXA1 (Rabbit Polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C. Concentration: 0.35 mg/mL Normal Rabbit IgG. One vial containing 125 µg Rabbit IgG in 125 µL storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75 μL of 5 μM of each primer specific for Mouse Hnf4α enhancer. Store at -20°C. FOR: TTC CAG CTG CCT TTA TCT CCC TGT REV: TCT CCA CAC ATG TCC AGC AGC CT Affinity purified |
Analysis Note【分析说明】 | Control Includes normal rabbit IgG and primers specific for Mouse Hnf4α enhancer. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, mouse |
manufacturer/tradename | ChIPAb+ Upstate® |
technique(s) | ChIP: suitable immunocytochemistry: suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_004487 |
UniProt accession no.【UniProt登记号】 | P55317 |
shipped in【运输】 | dry ice |
Gene Information | human ... FOXA1(3169) |
packaging【包装】 | 25 assays per set. Recommended use: 2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context). |
产品说明
Target description【目标描述】 | ~51 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |