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CD164 human
产品编号: | 4155563 |
规格: | recombinant, expressed in E. coli, 0.5 mg protein/mL |
包装规格: | 50 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
NACRES | NA.75 |
Application【应用】 | Coating a plate well (6 well plate) with this recombinant CD164 matrix protein in HSC cell specific medium at 1-10 μg/well allows for human HSC / receptor interaction studies in vitro. Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice. 1. Thaw CD164 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so the volume added covers the surface evenly (1-10 μg/well, 6 well plate). Note: Use 1 ml PBS per well in a 6-well plate. 2. Add 1 - 10 μg protein to each well and incubate at 2 to 10 ℃ overnight. 3. After incubation, aspirate remaining material. 4. Plates are ready for use. They may also be stored at 2-8 ℃ damp or air dried if sterility is maintained |
Sequence【序列】 | MASMTGGQQMGRGHHHHHHGNLYFQGGEFDKNTTQHPNVTTLAPISNVTSAPVTSLPLVTTPAPETCEGRNSCVSCFNVSVVNTTCFWIECKDESYCSHNSTVSDCQVGNTTDFCSVSTATPVPTANSTAKPTVQPSPSTTSKTVTTSGTTNNTVTPTSQPVRKSTFD |
Preparation Note【制备说明】 | The full-length extracellular domain of the human CD164 cDNA (24 - 162 aa) was constructed with 29 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified as soluble protein. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in E. coli |
description【描述】 | 0.05 mg of recombinant human CD164 in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol. |
sterility【无菌性】 | Filtered sterilized solution |
Assay【测定】 | ≥90% (SDS-PAGE) |
form【形式】 | liquid |
packaging【包装】 | pkg of 50 μg |
concentration【浓度】 | 0.5 mg protein/mL |
accession no.【登记号】 | NP_006007 |
UniProt accession no.【UniProt登记号】 | Q04900 |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... CD164(8763) |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |