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CD164 human

产品编号:4155563
规格:recombinant, expressed in E. coli, 0.5 mg protein/mL
包装规格:50 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415556350 μG3280
基本信息
NACRES
NA.75
Application【应用】
Coating a plate well (6 well plate) with this recombinant CD164 matrix protein in HSC cell specific medium at 1-10 μg/well allows for human HSC / receptor interaction studies in vitro.

Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CD164 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so the volume added covers the surface evenly (1-10 μg/well, 6 well plate).
Note: Use 1 ml PBS per well in a 6-well plate.
2. Add 1 - 10 μg protein to each well and incubate at 2 to 10 ℃ overnight.
3. After incubation, aspirate remaining material.
4. Plates are ready for use. They may also be stored at 2-8 ℃ damp or air dried if sterility is maintained
Sequence【序列】
MASMTGGQQMGRGHHHHHHGNLYFQGGEFDKNTTQHPNVTTLAPISNVTSAPVTSLPLVTTPAPETCEGRNSCVSCFNVSVVNTTCFWIECKDESYCSHNSTVSDCQVGNTTDFCSVSTATPVPTANSTAKPTVQPSPSTTSKTVTTSGTTNNTVTPTSQPVRKSTFD
Preparation Note【制备说明】
The full-length extracellular domain of the human CD164 cDNA (24 - 162 aa) was constructed with 29 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified as soluble protein.
产品性质
biological source【生物来源】
human
recombinant【重组】
expressed in E. coli
description【描述】
0.05 mg of recombinant human CD164 in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
sterility【无菌性】
Filtered sterilized solution
Assay【测定】
≥90% (SDS-PAGE)
form【形式】
liquid
packaging【包装】
pkg of 50 μg
concentration【浓度】
0.5 mg protein/mL
accession no.【登记号】
NP_006007
UniProt accession no.【UniProt登记号】
Q04900
storage temp.【储存温度】
−20℃
Gene Information
human ... CD164(8763)
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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