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Anti-ZO-1 Antibody, clone R40.76

产品编号:4155084
规格:clone R40.76, from rat
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4155084100 μG6190
产品别名

Anti-ZO-1 Antibody, clone R40.76

tight junction protein 1, zonula occludens 1 protein, Zona occludens protein 1, Zonula occludens protein 1, zona occludens 1

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
Specificity【特异性】
The antibody recognizes ZO-1.
Immunogen【免疫原】
Epitope: Unknown
Purified protein corresponding to mouse ZO-1.
Application【应用】
Research Category
Cell Structure
Anti-ZO-1 Antibody, clone R40.76 detects level of ZO-1 & has been published & validated for use in WB, IP, IH.
Research Sub Category
ECM Proteins
Immunofluorescence Analysis: A previous lot was used by an indepenedent laboratory in IF (D′Angelo Siliciano, 1988).
Quality【质量】
Evaluated by Western blot in mouse brain lysate.

Western blot Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 10 µg of mouse brain lysate.
Physical form【外形】
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified
Analysis Note【分析说明】
Control
Mouse brain lysate
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
rat
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
R40.76, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG2aκ
NCBI accession no.【NCBI登记号】
NP_033412
UniProt accession no.【UniProt登记号】
P39447
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
~220 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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