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Anti-ZO-1 Antibody, clone R40.76
产品别名
Anti-ZO-1 Antibody, clone R40.76
tight junction protein 1, zonula occludens 1 protein, Zona occludens protein 1, Zonula occludens protein 1, zona occludens 1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. |
Specificity【特异性】 | The antibody recognizes ZO-1. |
Immunogen【免疫原】 | Epitope: Unknown Purified protein corresponding to mouse ZO-1. |
Application【应用】 | Research Category Cell Structure Anti-ZO-1 Antibody, clone R40.76 detects level of ZO-1 & has been published & validated for use in WB, IP, IH. Research Sub Category ECM Proteins Immunofluorescence Analysis: A previous lot was used by an indepenedent laboratory in IF (D′Angelo Siliciano, 1988). |
Quality【质量】 | Evaluated by Western blot in mouse brain lysate. Western blot Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 10 µg of mouse brain lysate. |
Physical form【外形】 | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Format: Purified Protein G Purified |
Analysis Note【分析说明】 | Control Mouse brain lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | R40.76, monoclonal |
species reactivity | mouse |
species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_033412 |
UniProt accession no.【UniProt登记号】 | P39447 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~220 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |