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Anti-Versican Antibody, a.a. 535-598 of mouse versican
产品别名
Anti-Versican Antibody, a.a. 535-598 of mouse versican
GAG alpha Domain
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest. |
Specificity【特异性】 | Mouse versican GAG alpha domain |
Immunogen【免疫原】 | Epitope: a.a. 535-598 of mouse versican GST fusion protein containing amino acids 535-598 of mouse versican. |
Application【应用】 | Research Category Cell Structure Immunohistochemistry (formalin fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain. Western blotting: 0.5 μg/mL Immunoprecipitation Optimal working dilutions must be determined by the end user. This Anti-Versican Antibody, a.a. 535-598 of mouse versican is validated for use in IP, WB, IH(P) for the detection of Versican. Research Sub Category ECM Proteins |
Physical form【外形】 | Format: Purified Immunoglobulin was purified by affinity chromatography and is presented in PBS with no preservative Anti-GST has not been adsorbed. |
Analysis Note【分析说明】 | Control Immunohistochemistry Positive control: surrounding cartilage, neural tube / brainWestern blotting positive control: newborn mouse brain |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | mouse |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_004385.2 |
UniProt accession no.【UniProt登记号】 | P13611 |
shipped in【运输】 | dry ice |
Gene Information | human ... VCAN(1462) |
产品说明
Storage and Stability【储存及稳定性】 | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |