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Anti-UBR5 Antibody, clone 16G10.1

产品编号:4155051
规格:clone 16G10.1, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4155051100 μG4840
产品别名

Anti-UBR5 Antibody, clone 16G10.1

E3 ubiquitin-protein ligase UBR5, EC: 2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, HECT-type E3 ubiquitin transferase UBR5, Hyperplastic discs protein homolog, hHYD, Progestin-induced protein

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
E3 ubiquitin-protein ligase UBR5 (UniProt; O95071; also known as EC: 2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, HECT-type E3 ubiquitin transferase UBR5, Hyperplastic discs protein homolog, hHYD, Progestin-induced protein) is encoded by the UBR5 (also known as EDD, EDD1, HYD, KIAA0896) gene (Gene ID: 51366) in human. UBR5 is a widely expressed nuclear E3 ligase that is most abundant in testis and expressed at high levels in brain, pituitary and kidney. It is a component of the N-end rule pathway that recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. UBR5 is involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination and may play a role in control of cell cycle progression. It is shown to regulate DNA topoisomerase II binding protein (TopBP1) in the DNA damage response and may have a tumor suppressive function. It acts as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of Lys-63-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. URB5 contains a zinc-finger domain (aa 1177-1245), a poly(A)-binding protein C-terminal (PABC) domain(aa 2377-2454), and a (Homologous to the E6-AP Carboxyl Terminus (HECT) domain (aa 2462-2799). Two isoforms of UBR5 have been described that are produced by alternative splicing.
Specificity【特异性】
Clone 16G10.1 detects E3 ubiquitin-protein ligage UBR5 in human cells. It targets an epitope within 181 amino acids from the C-terminal half.
Immunogen【免疫原】
GST/His-tagged recombinant fragment corresponding to 181 amino acids from the C-terminal half of human E3 ubiquitin-protein ligase UBR5.
Application【应用】
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UBR5 in human cerebral cortex, human tonsil, and human prostate cancer tissues.

Anti-UBR5, clone 16G10.1, Cat. No. MABF1110, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase UBR5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Inflammation & Immunology
Quality【质量】
Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected UBR5 in 10 µg of Raji cell lysate.
Physical form【外形】
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
16G10.1, monoclonal
species reactivity
human
packaging【包装】
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype【同位素/亚型】
IgG2bκ
NCBI accession no.【NCBI登记号】
NP_056986
UniProt accession no.【UniProt登记号】
O95071
shipped in【运输】
ambient
Gene Information
human ... UBR5(51366)
产品说明
Target description【目标描述】
~305 kDa oserved; 309.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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