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Anti-TTBK1 Antibody, clone F287-1.1-1E9

产品编号:4155044
规格:clone F287-1.1-1E9, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4155044100 μG5520
产品别名

Anti-TTBK1 Antibody, clone F287-1.1-1E9

Tau-tubulin kinase 1, Brain-derived tau kinase

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Tau-tubulin kinase 1 (EC 2.7.11.1; UniProt Q5TCY1; also known as Brain-derived tau kinase) is encoded by the TTBK1 (also known as BDTK, KIAA1855) gene (Gene ID 84630) in human. Tau-tubulin kinase 1 and 2 (TTBK1 and TTBK2) belong to the casein kinase 1 superfamily of kinases. TTBK2 is ubiquitously expressed, while TTBK1 is neuron-specific and can directly phosphorylate tau proteins at multiple Ser/Thr residues that are found on paired helical filament-tau or PHF-tau in Alzheimer′s disease (AD) brains. Consistently, transgenic mice over-expressiong human TTBK1 develop age-dependent pathology in brain, including intraneuronal accumulation of phosphorylated neurofilament (p-NF), tau phosphorylation, cortical and hippocampal astro/microgliosis, calpain I up-regulation, enhanced CDK5/p35 complex formation, down regulation of NMDA receptor, and memory impairment.
Specificity【特异性】
Expected to react with both spliced isoforms reported by UniProt (Q5TCY1-1 and Q5TCY1-2). Clone F287-1.1-1E9 detected the full-length as well as the proteolytically processed human and mouse TTBK1 (Sato, S., et al. (Xu, J., et al. (2010). FASEB J. 24(8):2904-2915; 2008). J. Neurosci. 28(53):14511-14521).
Immunogen【免疫原】
Epitope: Kinase domain.
His-tagged recombinant fragment within the kinase domain of human TTBK1.
Application【应用】
Immunohistochemistry Analysis: A representative lot detected a much higher TTBK1 immunoreactivity in paraformaldehyde-fixed frozen hippocampus and cortex sections from mice with human TTBK1 transgene (Xu, J., et al. (2010). FASEB J. 24(8):2904-2915).
Immunohistochemistry Analysis: A representative lot detected a much higher TTBK1 immunoreactivity in the subiculum and external pyramidal layer of visual cortex in paraformaldehyde-fixed frozen brain sections from transgenic mice overexpressing human TTBK1 (Sato, S., et al. (2008). J. Neurosci. 28(53):14511-14521).
Western Blotting Analysis: Representative lots detected both the full-length and the proteolytically processed murine TTBK1 in tissues (cerebellum, cortex, spinal cord) from wild-type mice, as well as the expression of human TTBK1 transgene in tissues from transgenic mice (Sato, S., et al. (Xu, J., et al. (2010). FASEB J. 24(8):2904-2915; 2008). J. Neurosci. 28(53):14511-14521).
Western Blotting Analysis: A representative lot detected an upregulation of both the full-length and the proteolytically processed TTBK1 in the frontal cortex gray matter extracts from Alzheimer′s diseased (AD) human brains than age-matched control non-AD brains (Sato, S., et al. (2008). J. Neurosci. 28(53):14511-14521).
Research Category
Neuroscience
Detect TTBK1 using this Anti-TTBK1 Antibody, clone F287-1.1-1E9 validated for use in Western Blotting, Immunohistochemistry.
Research Sub Category
Neurodegenerative Diseases
Quality【质量】
Evaluated by Western Blotting in TTBK1 trangenic mouse brain tissue lysate.

Western Blotting Analysis: 2.5 µg/mL of this antibody detected the expression of human TTBK1 transgene in 20 µg of transgenic mouse brain tissue lysate.
Physical form【外形】
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
F287-1.1-1E9, monoclonal
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype【同位素/亚型】
IgG2aκ
NCBI accession no.【NCBI登记号】
NP_115927
UniProt accession no.【UniProt登记号】
Q5TCY1
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
~142 observed. 142.7 kDa (isoform 1) and 69.08 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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