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Anti-TorsinB Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | TorsinB. The antibody reacts with the ~38 kDa protein. |
Immunogen【免疫原】 | Synthetic peptide from torsinB. |
Application【应用】 | Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC. Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment. Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA. Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G. Optimal working dilutions must be determined by end user. |
Analysis Note【分析说明】 | Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
Legal Information【法律信息】 | Control Western blot: brain and liver. Immunocytochemistry: SH-SY5Y cells. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | rat, mouse, human |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_014506.1 |
UniProt accession no.【UniProt登记号】 | O14657 |
shipped in【运输】 | dry ice |
Gene Information | human ... TOR1B(27348) |
产品说明
Storage and Stability【储存及稳定性】 | Serum |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |