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Anti-TIMP-3 Antibody, CT
产品编号: | 4155027 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-TIMP-3 Antibody, CT
MIG-5, TIMP metallopeptidase inhibitor 3, Tissue inhibitor of metalloproteinases 3
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Tissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix. |
Specificity【特异性】 | The antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2. |
Immunogen【免疫原】 | Epitope: C-terminus KLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus. |
Application【应用】 | Research Category Cell Structure This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells. Research Sub Category MMPs & TIMPs |
Quality【质量】 | Evaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media. Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media. |
Linkage【联系】 | Replaces: AB802 |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control HL-60 cell lysate in PMA-conditioned media. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, horse, rat |
species reactivity (predicted by homology) | bovine (based on 100% sequence homology), equine (based on 100% sequence homology), mouse (95% homology), pig (based on 100% sequence homology), primate (based on 100% sequence homology), canine (based on 100% sequence homology), monkey (based on 100% sequence homology) |
technique(s) | immunocytochemistry: suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_000353 |
UniProt accession no.【UniProt登记号】 | P35625 |
shipped in【运输】 | wet ice |
Gene Information | human ... TIMP3(7078) |
产品说明
Target description【目标描述】 | Bands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |