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Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2
产品别名
Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2
Synaptotagmin-like protein 4, Exophilin-2, Granuphilin
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Synaptotagmin-like protein 4 (UniProt Q96C24; also known as Exophilin-2, Granuphilin) is encoded by the SYTL4 (also known as SLP4) gene (Gene ID 94121) in human. SYLT4 belongs to the synaptotagmin-like protein family, which contains an N-terminal RabBD (Rab-binding) domain and two C-terminal C2 domains. The characteristic feature of this family is the N-terminal domain, which is not found in other C-type tandem C2 proteins. SYLT4 is detected close to the plasma membrane and on secretory granules. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner. It can bind to both the GTP- and GDP-bound forms of Rab27A and inhibits a specific GTP/GDP exchange cycle required for dense-core vesicle exocytosis. It is the first regulatory in the exocytosis pathway that functions by directly connecting Rab and SNARE. It modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. It is not only essential for the docking of insulin granules, but also simultaneously imposes a fusion constraint on them through an interaction with syntaxin-1alpha fusion machinery. |
Specificity【特异性】 | Clone 7D2.2 epitope resides within an internal region mostly missing in human spliced isoform 2. Reactivity toward isoform 2 has not been confirmed. |
Immunogen【免疫原】 | GST-tagged recombinant human synaptotagmin-like protein 4 (SYTL4) internal fragment. |
Application【应用】 | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of human adrenal gland tissue lysate. Detect SYTL4 using this mouse monoclonal Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2, Cat. No. MABS1713, validated for use in Western Blotting. Research Category Signaling |
Quality【质量】 | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of HeLa cell lysate. |
Physical form【外形】 | Protein G purified. Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 7D2.2, monoclonal |
species reactivity | human |
technique(s) | western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_001123368 |
UniProt accession no.【UniProt登记号】 | Q96C24 |
shipped in【运输】 | ambient |
Gene Information | human ... SYTL4(94121) |
产品说明
Target description【目标描述】 | ~80 kDa observed. 70.02 kDa (human isoform 1) calculated. Uncharacterized bands may be observed in some lysate(s). |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |