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Anti-Signal peptide peptidase-like 3 Antibody, clone 7F9
产品别名
Anti-Signal peptide peptidase-like 3 Antibody, clone 7F9
Signal peptide peptidase-like 3, SPP-like 3, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Signal peptide peptidase-like 3 (UniProt: Q8TCT6; also known as SPP-like 3, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4, SPPL3) is encoded by the SPPL3 (also known as IMP2) gene (Gene ID: 121665) in human. SPPL3 is a multi-pass transmembrane protein that is shown to be widely expressed and is highly conserved in multicellular eukaryotes. Three isoforms of SPPL3 have been described that are produced by alternative splicing. It is a member of the intramembrane-cleaving GxGD proteases. It acts as an intramembrane protease that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries. It can also act as a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifying glycosidases and glycosyltransferases. It is also shown to catalyze the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase-mediated cleavage proteolysis. The first transmembrane domain of SPPL3 may act as a type I signal anchor. SPPL3 also contains a PAL motif (aa 342-344) that is required for normal active site conformation. |
Specificity【特异性】 | Clone 7F9 detects Signal peptide peptidase-like 3 in human cells. it targets an epitope within 15 amino acids from the C-terminal half. Immunogen sequence is conserved in all three isoforms. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human Signal peptide peptidase-like 3. |
Application【应用】 | Western Blotting Analysis: A representative lot detected Signal peptide peptidase-like 3 in Western Blotting applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9; Voss, M., et. al. (2014). EMBO J. 33(24):2890-905; Fleck, D., et. al. (2016). J Biol Chem. 291(1):318-33). Immunoprecipitation Analysis: A representative lot detected Signal peptide peptidase-like 3 in Immunoprecipitation applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9). Anti-Signal peptide peptidase-like 3, clone 7F9, Cat. No. MABS1910, is a mouse monoclonal antibody that detects Signal peptide peptidase-like 3 and has been tested for use in Immunoprecipitation and Western Blotting. Research Category Signaling |
Quality【质量】 | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Signal peptide peptidase-like 3 in HEK293 cell lysate. |
Physical form【外形】 | Protein G purified Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 7F9, monoclonal |
species reactivity | human |
packaging【包装】 | antibody small pack of 25 μg |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1κ |
NCBI accession no.【NCBI登记号】 | NP_620584 |
UniProt accession no.【UniProt登记号】 | Q8TCT6 |
shipped in【运输】 | ambient |
Gene Information | human ... SPPL3(121665) |
产品说明
Target description【目标描述】 | ~42 kDa observed; 42.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |