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Anti-SH2D1A Antibody, clone SuGr-20 18A9
产品别名
Anti-SH2D1A Antibody, clone SuGr-20 18A9
SH2 domain-containing protein 1A, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | SH2 domain-containing protein 1A (UniProt O60880; also known as Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP) is encoded by the SH2D1A (also known as DSHP, SAP, XLP, XLP1, XLPD) gene (Gene ID 4068) in human. SAP/SH2D1A is a 628-amino acid (isoform A) adaptor protein expressed in T cells, natural killer (NK) cells, NK-T cells, and some B cells. SAP/SH2D1A is made up almost exclusively of a Src homology 2 (SH2) domain (a.a. 6-104) and plays an important role in multiple immune cell functions, including T helper 2 (TH2) cytokine production, T cell-dependent antibody production, follicular T helper (TFH) cell differentiation, NK-T cell development, CD8+ T cell- and NK cell-mediated cytotoxicity. SAP couples signaling lymphocytic activation molecule (SLAM) family receptors to tyrosine phosphorylation signals through its interaction with the SH3 domain of Src family protein tyrosine kinase Fyn. In addition, SAP can prevent SH2 domain-containing negative regulators, such as phosphatases SHIP-1 and SHP-1, from inactivating SLAM-related receptors by blocking their interaction with the receptors. Evidence also shows that SAP/SH2D1A regulates T cell-dependent antibody production and TFH cell differentiation independent of Fyn. SAP/SH2D1A gene is mutated and inactivated in X-linked lymphoproliferative disease, a rare human immunodeficiency characterized by an uncontrolled immune response to Epstein-Barr virus infection. |
Specificity【特异性】 | Clone SuGr-20 18A9 recognizes recombinant full-length human SH2D1A spliced isoform A (UniProt O60880-1) by Western blotting. Reactivity toward spliced isoforms B to F (UniProt O60880-2 to O60880-6) has not been determined. |
Immunogen【免疫原】 | Recombinant full-length human SH2D1A. |
Application【应用】 | Research Category Signaling Research Sub Category Immunological Signaling Anti-SH2D1A Antibody, clone SuGr-20 18A9 is an antibody against SH2D1A for use in Western Blotting. |
Quality【质量】 | Evaluated by Western Blotting of recombinant human SH2D1A. Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.1 µg of recombinant human SH2D1A. |
Physical form【外形】 | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | SuGr-20 18A9, monoclonal |
species reactivity | human |
technique(s) | western blot: suitable |
isotype【同位素/亚型】 | IgG2bκ |
NCBI accession no.【NCBI登记号】 | NP_002342 |
UniProt accession no.【UniProt登记号】 | O60880 |
shipped in【运输】 | wet ice |
Gene Information | human ... SH2D1A(4068) |
产品说明
Target description【目标描述】 | ~14 kDa observed. 14.19 kDa calculated. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |