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Anti-Septin-3 Antibody, clone 5C1.2

产品编号:4154922
规格:clone 5C1.2, from mouse
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154922100 μL5350
产品别名

Anti-Septin-3 Antibody, clone 5C1.2

Neuronal-specific septin-3E

基本信息
eCl@ss
32160702
General description【一般描述】
Neuronal-specific septin-3 (UniProt Q9UH03) is encoded by the SEPT3 (also known as SEP3) gene (Gene ID 55964) in human. Septins are GTPases capable of forming higher-order oligomers and filamentous polymers that contact with cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. Septins exist in complexes that are composed of ordered non-polar arrays of multiple different septins. Human genome contains 13 septin genes that are grouped into the SEPT2 subgroup (septins 1, 2, 4, and 5), the SEPT3 subgroup (septins 3, 9, and 12), the SEPT6 subgroup (septins 6, 8, 10, 11, and 14), and the SEPT7 subgroup (septin 7). All subgroups contain the well conserved GTP-binding domain and differ mainly in their C-terminal region, where coiled-coil sequences are found in SEPT2, SEPT6, and SEPT7, but not SEPT3, subgroups. Septin-3 is the only septin to be expressed almost exclusively in brain tissue, where it is enriched in nerve terminals, suggesting a role in synaptic vesicle function. Increased septin-3 expression is reported in several brain tumours and SEPT3 polymorphisms are linked to Alzheimer′s disease. PKG-1 is shown to phosphorylate septin-3 in vitro, non-phosphorylated septin-3 localizes to the peripheral membrane, whereas the phosphorylated form is found in the cytosol. Murine and rat septin-3 is reported to form complexes with septin-5 and septin-7.
Specificity【特异性】
Clone 5C1.2 targets an internal epitope present in human spliced isoforms 1&2 (SEP3A and SEP3B), both mouse and all three rat spliced isoforms (G-septin-alpha, -beta, and -gamma) reporeted by UniProt (Q9UH03, Q9Z1S5, and Q9WU34, respectively). Target epitope is absent from human spliced isoform 3 (SEP3C).
Immunogen【免疫原】
KLH-conjugatged linear peptide coresponding to an internal sequence from the N-terminal half of human septin-3.
Application【应用】
Western Blotting Analysis: A 1:1,000 dilution of from a representative lot detected Septin-3 in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Septin-3 in human cerebellum and cerebral cortex, as well as in mouse cerebral cortex tissue sections.

Research Category
Neuroscience
Anti-Septin-3, clone 5C1.2, Cat. No. MABN1153, is a highly specific mouse monoclonal antibody that targets Septin-3, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Quality【质量】
Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Septin-3 in 10 µg of mouse brain tissue lysate.
Physical form【外形】
Protein G purified.
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
5C1.2, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NP_663786
UniProt accession no.【UniProt登记号】
Q9UH03
shipped in【运输】
ambient
Gene Information
human ... SEPT3(55964)
产品说明
Target description【目标描述】
~40/39 kDa observed. 40.70/40.10 kDa (human isoform 1/2), 40.04/38.71 kDa (mouse isoform 1/2), and 40.60/39.62/34.30 kDa (rat isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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