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Anti-Rabies Virus CVS-11 Antibody, clone 509-6
产品别名
Anti-Rabies Virus CVS-11 Antibody, clone 509-6
Glycoprotein, Glycoprotein Rabies virus, Rabies virus strain CVS-11
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Glycoprotein (UniProt: O92284) is encoded by the G gene in Rabies virus, strain CVS-11. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone 509-6 is shown to neutralize all fixed and street rabies virus strains except some virus isolates from bats. The conformational antigenic site I for this clone is located at aa 231. (Ref.: Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672 4678). |
Specificity【特异性】 | Clone 509-6 detected glycoprotein from Rabies virus, strain CVS-11 in L929 cells expressing the rabies protein. It did not detect glycoprotein in wild type L929 cells. |
Immunogen【免疫原】 | Beta-propiolactone inactivated Rabies virus, strain CVS-11 (RABV). |
Application【应用】 | Neutralizing Analysis: A representative lot neutralized Rabies Virus (Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5; Bunschoten, H., et. al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8). ELISA Analysis: A representative lot detected Rabies Virus in ELISA applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8). Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Rabies Virus in Fluorescence Activated Cell Sorting (FACS) applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8). Anti-Rabies Virus, clone 509-6, Cat. No. MABF2073, is a mouse monoclonal antibody that detects glycoprotein of Rabies virus, strain CVS-11 and has been tested for use in ELISA, Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), and Neutralizing. Research Category Inflammation & Immunology |
Quality【质量】 | Evaluated by Flow Cytometry in L929 cells expressing the rabies protein and in L929 (WT) cells. Flow Cytometry Analysis: 1 µg of this antibody detected glycoprotein of Rabies virus in one million L929 cells expressing the rabies protein. |
Physical form【外形】 | Protein G purified Format: Purified Purified mouse monoclonal antibody IgG2a in PBS without azide. |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 509-6, monoclonal |
species reactivity | rabies virus |
species reactivity (predicted by homology) | all |
packaging【包装】 | antibody small pack of 25 μg |
technique(s) | ELISA: suitable flow cytometry: suitable neutralization: suitable |
isotype【同位素/亚型】 | IgG2aκ |
UniProt accession no.【UniProt登记号】 | O92284 |
shipped in【运输】 | ambient |
产品说明
Target description【目标描述】 | 58.86 kDa calculated. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |