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Anti-R-Spondin 2 Antibody, clone 14C7.1
产品别名
Anti-R-Spondin 2 Antibody, clone 14C7.1
Roof plate-specific spondin-2, hRspo2
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | R-spondin-2 (UniProt: Q6UXX9; also known as Roof plate-specific spondin-2, hRspo2) is encoded by the RSPO2 (also known as UNQ9384/PRO34209) gene (Gene ID: 340419) in human. R-spondin-2 is a secreted protein that acts as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR receptors. Upon binding to LGR receptors (LGR4, LGR5 or LGR6) these receptors associate with phosphorylated LRP6 and frizzled receptors, which are activated by extracellular Wnt receptors. These events trigger the canonical Wnt signaling pathway to increase expression of target genes. R-spondin-2 also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling pathway by acting as an inhibitor of ZNRF3, an E3 ubiquitin-protein ligase that serves as an important regulator of the Wnt signaling pathway. In humans, R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lungs and is reported to downregulated in tumors involving these organs. In the embryonic mice it is abundantly expressed in the hippocampus and in developing bones. |
Specificity【特异性】 | Clone 14C7.1 specifically detects human R-spondin-2. It targets an epitope within 74 amino acids from the C-terminal end. |
Immunogen【免疫原】 | GST/His-tagged recombinant fragment corresponding to 74 amino acids from the C-terminal end of human R-Spondin 2. |
Application【应用】 | Anti-R-Spondin 2, clone 14C7.1, Cat. No. MABS1709, is a mouse monoclonal antibody that specifically targets R-Spondin 2 (RSPO2) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. Research Category Signaling Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected RSPO2 in human pancreas, human kidney, and human bone marrow tissue. |
Quality【质量】 | Evaluated by Western Blotting in human recombinant R-spondin 2 protein. Western Blotting Analysis: 0.02 µg/mL of this antibody detected 0.05 µg of human recombinant R-spondin 2 protein. |
Physical form【外形】 | Protein G purified Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 14C7.1, monoclonal |
species reactivity | human |
packaging【包装】 | antibody small pack of 25 μg |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
isotype【同位素/亚型】 | IgG2bκ |
NCBI accession no.【NCBI登记号】 | NP_848660 |
UniProt accession no.【UniProt登记号】 | Q6UXX9 |
shipped in【运输】 | ambient |
Gene Information | human ... RSPO2(340419) |
产品说明
Target description【目标描述】 | ~35 kDa observed; 28.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |