Anti-Polycystin-L Antibody

32160702

NA.41

Polycystin-L

Synthetic peptide from the N-terminal of human Polycystin-L1.

Research Sub Category
Renal Physiology
Research Category
Metabolism
Western blot: 1:1,000 using ECL. The antibody reacts with the 90 kDa Polycystin1-L1 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.
This Anti-Polycystin-L Antibody is validated for use in WB, IC, IH for the detection of Polycystin-L.

Control
POSITIVE CONTROL: adult mouse retina

NEGATIVE CONTROL: adult mouse lung

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

mouse

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NM_138295.2

Q9P0L9

dry ice

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1