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Anti-Peripherin Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Peripherin is a 56-58 kDa type III intermediate filament protein (Portier et al. 1983), expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g. Hirschsprung′s disease) states. Abnormal inclusions containing peripherin have also been reported in ALS. During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness. |
Specificity【特异性】 | Strong staining on rat and human. Cross-reacts with mouse, pig, and bovine. Other species not tested. Recognizes Peripherin. The antibody stains a ~57 kDa band cleanly and specifically and does not stain vimentin, GFAP, alpha-internexin or any of the neurofilament subunits. Strong staining on rat, mouse, human, pig and cow peripherin. Does not stain chicken, quail or other more distantly related species which appear to lack peripherin. |
Immunogen【免疫原】 | Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990) 57:235-248], containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE. |
Application【应用】 | Research Sub Category Sensory & PNS Neuronal & Glial Markers Additional Research Applications Immunohistochemistry: A previous lot of this antibody was used at 1:100-1:200 dilution. It is suggested that the antibody be used on frozen sections fixed in acetone at -20°C. AB1530 will work on tissue fixed for one hour or less in fresh 4% paraformaldehyde. It has been reported that this antibody can be used on paraffin embedded tissue sections. See Cell & Tissue Research (1997) 288:11-23 & European J. Dermatology (1998) 8:339-342. Electron Microscopy: A previous lot of this antibody was used on Electron Microscopy. Optimal working dilutions and protocols must be determined by end user. This Anti-Peripherin Antibody is validated for use in IH, IH(P), WB for the detection of Peripherin. Research Category Neuroscience |
Quality【质量】 | Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:1000 dilution of this lot detected peripherin on 10 μg of PC12 lysates. |
Physical form【外形】 | Rabbit polyclonal antisera, no preservatives. Unpurified |
Analysis Note【分析说明】 | Control Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | bovine, human, mouse, pig, rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_006253 |
UniProt accession no.【UniProt登记号】 | P41219 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 56-58 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |