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Anti-pan-ADP-ribose binding reagent
产品别名
Anti-pan-ADP-ribose binding reagent
pan-ADP-ribose binding reagent
基本信息
eCl@ss | 32160405 |
General description【一般描述】 | Cat. No.MABE1016, Anti-pan-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta |
Specificity【特异性】 | poly(ADP-ribose) and mono(ADP-ribose) |
Application【应用】 | Anti-poly-ADP-ribose binding reagent is a reagent that selectively binds to both mono- and poly- ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot. Research Sub Category General Post-translation Modification Dot Blot Specificity Analysis: This reagent detected mono(ADPR) on recombinant PARP3 protein, as well as mono(ADPR) and poly(ADPR) on recombinant PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center). Immunoprecipitation Analysis: A representative lot of Anti-pan-ADP-ribose binding reagent immunoprecipitated ADP-ribosylated proteins from nuclear extract (Lee Kraus, University of Texas Southwestern). Western Blotting Analysis: A representative lot detected auto-ADP-ribosylation activity of PARP1/2/3 and mutants in the presence of NAD+ or various NAD+ analogs (Gibson, B.A., et al. (2016). Science. 353(6294):45-50). Western Blotting Analysis: A representative lot detected PARP1-catalyzed NELF-E ADP-ribosylation in cell-free enzymatic reactions as well as ADP-ribosylation of exogenously expressed FLAG-tagged NELF-E in HEK293T cells. PARP inhibitor PJ34 or P-TEFb/CDK9 inhibitor flavopiridol treatment decreased cellular NELF-E ADP-ribosylation level (Gibson, B.A., et al. (2016). Science. 353(6294):45-50). Research Category Epigenetics & Nuclear Function |
Quality【质量】 | Evaluated by Western Blotting on ADP-ribosylated PARP1 and PARP3 recombinant proteins. Western Blotting Analysis: This reagent detected mono(ADPR) on recombinant PARP3 protein, as well as mono(ADPR) and poly(ADPR) on recombinant PARP1 protein (Lee Kraus, University of Texas Southwestern Medical Center). |
Physical form【外形】 | Format: Purified Ni-NTA agarose Purified from E. coli by Ni-NTA agarose. Supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10% Glycerol, 10 mM Imidazole, 1 mM PMSF, 1 mM β-Mercaptoethanol, 10% glycerol without preservatives. |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
biological source【生物来源】 | Escherichia coli |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
species reactivity | human, mouse |
species reactivity (predicted by homology) | all |
technique(s) | dot blot: suitable immunoprecipitation (IP): suitable western blot: suitable |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | Variable depending on the target proteins and the extend of ADP-ribosylation. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |