您的位置:首页 > 产品中心 > Anti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1
Anti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1
产品别名
Anti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1
Nucleobindin-2, Nestafin-1, DNA-binding protein NEFA, Prepronesfatin, Pronesfatin
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Nucleobindin-2 (UniProt Q9JI85; also known as DNA-binding protein NEFA, Prepronesfatin) is encoded by the Nucb2 (also known as Nefa) gene (Gene ID 59295) in rat species. Nucleobindin-2 is initially translated as a 420-a.a. precursor protein. Cleavage of signal peptide (aa1-24) yields the 396-a.a. mature nucleobindin-2 with very high sequence homology among human, mouse, and rat species (more than 85%). Post-translational processing by prohormone convertases (PC 1/3 and PC 2) cleaves nucleobindin-2 into nesfatin-1, nesfatin-2, and nesfatin-3 (aa1–82, aa85–163, and aa166–396, respectively, numbering based on mature sequence). Nesfatin-1 is a well recognized circulating anorexigenic peptide, whose production increases post-prandially. Nucleobindin-2 contains two calcium-binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper. Nucleobindin-2 is known to be expressed in key hypothalamic nuclei and plays a role in energy homeostasis. Nucleobindin-2 is also expressed in various human peripheral tissues, including the stomach, pancreas, reproductive organs, and adipose tissues, suggesting roles in various metabolic functions. Nucleobindin-2 expression level is reported to be elevated in clear cell renal cell carcinoma (ccRCC), breast cancer, and prostate cancer (PCa) tissues. |
Specificity【特异性】 | Clone 3B3.1 reacts with pro-nucleobindin-2, mature nucleobindin-2, and nesfatin-1, but not nesfatin-2 or nesfatin-3. |
Immunogen【免疫原】 | Epitope: Nesfatin-1 GST-tagged recombinant rat Nestafin-1. |
Application【应用】 | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected nucleobindin-2/nestafin-1 immunoreactivity in human colon cancer tissue, as well as in normal testis, tonsil, and pancreas tissue sections. Research Sub Category Metabolic Hormones & Receptors Research Category Signaling This Anti-Nucleobindin-2 Antibody, clone 3B3.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Nestafin-1. |
Quality【质量】 | Evaluated by Western Blotting in mouse liver tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected nucleobindin-2 in 10 µg of mouse liver tissue lysate. |
Physical form【外形】 | Protein G purified Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 3B3.1, monoclonal |
species reactivity | mouse, human, rat |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
isotype【同位素/亚型】 | IgG1κ |
NCBI accession no.【NCBI登记号】 | NP_067695 |
UniProt accession no.【UniProt登记号】 | Q9JI85 |
产品说明
Target description【目标描述】 | ~50 kDa observed. 47.33/50.20 kDa (human mature/pro-nucleobindin-2), 47.42/50.30 kDa (mouse mature/pro-nucleobindin-2), 47.25/50.09 kDa (rat mature/pro-nucleobindin-2), 9.552/9.612/9.583 kDa (human/mouse/rat nesfatin-1) calculated. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |