Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat

32160702

NA.41

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Strong reactivity to the major neurofilament subunit HF-H. Since a second neurofilament subunit, NF-M, also contains a few lysine-serine-proline (KSP) sequences, there is generally some reactivity with this protein also. AB1991 stains both phosphorylated and dephosphorylated neurofilaments.

Epitope: lysine-serine-proline repeat
E. Coli recombinant fusion protein containing 37 lysine-serine-proline repeats of rat NF-H.

Detect Neurofilament H (200 kDa) using this Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat validated for use in IH(P), WB, IH.
Research Category
Neuroscience
Immunohistochemistry: 1:250-1:1,000 AB1991 is not affected by the level of neurofilament phosphorylation and is particularly good for revealing dendritic and perikaryal neurofilaments.

Immunoblotting

Electron microscopy

Optimal working dilutions must be determined by end user.
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Neat rabbit antisera.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

avian, reptile

mammals

Chemicon^®

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NM_021076.2

P12036

dry ice

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

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10 - Combustible liquids

WGK 1