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Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485]

产品编号:4154662
规格:Chemicon®, from rabbit
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154662100 μL5420
产品别名

Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485]

Neural Wiskott-Aldrich Syndrome Protein

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.
Specificity【特异性】
The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.
Immunogen【免疫原】
Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.
Epitope: phosphoserine484/phosphserine485
Application【应用】
Detect N-WASP using this Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485] validated for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Western blot: 1:1000 ELISA: 1:2000
Physical form【外形】
This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide (without carrier) then affinity purified using phospho-N-WASP (S484/S485) peptide (without carrier). Antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NM_003941.2
UniProt accession no.【UniProt登记号】
O00401
shipped in【运输】
wet ice
Gene Information
human ... WASL(8976)
产品说明
Storage and Stability【储存及稳定性】
Maintain at 20°C for up to one year from date of receipt. Do not aliquot.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
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