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Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485]
产品别名
Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485]
Neural Wiskott-Aldrich Syndrome Protein
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types. |
Specificity【特异性】 | The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP. |
Immunogen【免疫原】 | Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484. Epitope: phosphoserine484/phosphserine485 |
Application【应用】 | Detect N-WASP using this Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485] validated for use in ELISA & WB. Research Category Signaling Research Sub Category Cytoskeletal Signaling Western blot: 1:1000 ELISA: 1:2000 |
Physical form【外形】 | This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide (without carrier) then affinity purified using phospho-N-WASP (S484/S485) peptide (without carrier). Antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, rat |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_003941.2 |
UniProt accession no.【UniProt登记号】 | O00401 |
shipped in【运输】 | wet ice |
Gene Information | human ... WASL(8976) |
产品说明
Storage and Stability【储存及稳定性】 | Maintain at 20°C for up to one year from date of receipt. Do not aliquot. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |