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Anti-mTOR Antibody, clone F11

产品编号:4154647
规格:clone F11, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154647100 μG5420
产品别名

Anti-mTOR Antibody, clone F11

FK506 binding protein 12-rapamycin associated protein 1, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated prot

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
Immunogen【免疫原】
GST-tagged recombinant protein corresponding to rat mTOR.
Application【应用】
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Nemazanyy, I, et al. (2008). Hybridoma, 27:395-398.)
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
This Anti-mTOR Antibody, clone F11 is validated for use in WB, IP for the detection of mTOR.
Quality【质量】
Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on 10 µg of HeLa cell lysate.
Physical form【外形】
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G
Analysis Note【分析说明】
Control
HeLa cell lysate
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
F11, monoclonal
species reactivity
human
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NP_063971
UniProt accession no.【UniProt登记号】
P42346
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
~ 289 kDa observed
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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