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Anti-MMP-2 Antibody, catalytic domain

产品编号:4154627
规格:Chemicon®, from rabbit
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154627100 μG6190
产品别名

Anti-MMP-2 Antibody, catalytic domain

Gelatinase A, 72 kDa Type IV Collagenase

基本信息
eCl@ss
32160702
NACRES
NA.41
Specificity【特异性】
Recognizes rat MMP-2. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain) as an immunogen.
Immunogen【免疫原】
E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain)
Epitope: catalytic domain
Application【应用】
Research Category
Cell Structure
Western blot: 1:2,000

Immunoprecipitation: 1:500

MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.

Optimal working dilutions must be determined by end user.
Detect MMP-2 using this Anti-MMP-2 Antibody, catalytic domain validated for use in IP & WB.
Research Sub Category
MMPs & TIMPs
Linkage【联系】
Replaces: 04-1048
Physical form【外形】
Purified immunoglobulin. Liquid in PBS, containing 0.05% sodium azide.
Format: Purified
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
human, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NM_004530.2
UniProt accession no.【UniProt登记号】
P08253
shipped in【运输】
wet ice
Gene Information
human ... MMP2(4313)
产品说明
Storage and Stability【储存及稳定性】
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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