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Anti-Mitochondrial Pyruvate Carrier 2 (BRP44)

产品编号:4154620
规格:clone n/a, from mouse
包装规格:25 μG,100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415462025 μG1550
4154620100 μG4510
产品别名

Anti-Mitochondrial Pyruvate Carrier 2 (BRP44)

Mitochondrial pyruvate carrier 2, Brain protein 44, BRP44

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Mitochondrial pyruvate carrier 2 (UniProt: O95563; also known as Brain protein 44, MPC2) is encoded by the MPC2 (also known as BRP44) in human. MPC2 is a multi-pass inner mitochondrial membrane protein that mediates the imports pyruvate into mitochondria. Pyruvate is an essential component in intermediary metabolism that occupies a key crossroads position between cytosolic glycolysis and mitochondrial metabolism. MPC2 contains 3 helical domains (aa 41-61; 73-90; and 96-115). In mammalian cells mitochondrial pyruvate carrier is composed on two paralogous subunits known as MPC1 and MPC2 that heteromerize to form a functional complex. It has been reported that MPC2 can also homomerize, however only MPC1/MPC2 heteromeric forms are functional. Complete absence of MPC2 is shown to cause embryonic lethality in murine models, however, murine cell lines that express an N-terminal truncated MPC2 protein (Mpc2 16) are reported to be viable, but exhibit significantly reduced capacity for mitochondrial pyruvate oxidation. (Ref.: Compan, V., et al. (2015). Mol. Cell 59(3); 491-501; Vigueira, PA., et al. (2014). Cell Rep. 7(6); 2042-2053).
Specificity【特异性】
This mouse monoclonal antibody detects Mitochondrial Pyruvate carrrier Protein 2 in human and mouse cells. It targets an epitope within 10 amino acids from the internal region.
Immunogen【免疫原】
A linear peptide corresponding to 10 amino acids from the internal region of human mitochondrial pyruvate carrier 2 protein.
Application【应用】
Anti-Mitochondrial Pyruvate Carrier 2 (BRP44), Cat. No. MABS1914, is a mouse monoclonal antibody that detects human and mouse Mitochondrial pyruvate carrier 2 and has been tested for use in Immunoprecipitation and Western Blotting.
Western Blotting Analysis: 1 µg/mL from a representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse heart and mouse liver tissue lysates.

Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland)..

Western Blotting Analysis: 4 µg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate.

Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056).

Research Category
Signaling
Quality【质量】
Evaluated by Western Blotting in mouse liver mitochondrial lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitochondrial lysate.
Physical form【外形】
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
n/a, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
packaging【包装】
antibody small pack of 25 μg
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NP_001137146
UniProt accession no.【UniProt登记号】
O95563
shipped in【运输】
ambient
产品说明
Target description【目标描述】
~15 kDa observed; 14.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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