Anti-LIMD1 Antibody, clone 3F2/C6

32160702

NA.41

LIMD1 (LIM domains-containing protein 1) is a tumor suppressor and member of the Ajuba family of LIM domain-containing proteins. LIMD1 interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Recently, LIMD1 has been implicated in regulating cellular processes involved in hypoxia. LIMD1 acts as a molecular scaffold, simultaneously binding the prolyl hydroxylases (PHDs) and von Hippel Lindau tumor suppressors (VHL), thereby assembling a PHD-LIMD1-VHL protein complex and creating an enzymatic niche that enables efficient degradation of HIF-1α.

This antibody recognizes human LIMD1.

Recombinant protein corresponding to human LIMD1

This antibody has been shown to work in Immunocytochemical applications (Foxler, D., et al. (2012).
This antibody has been shown to work in Immunohistochemical applications (Sharp, T., et al. (2008).
This antibody has been shown to work in Immunoprecipitation applications (Courtesy of Dr. Tyson Sharp, Cancer Research, UK)
Research Sub Category
Oncoproteins

Oxidative Stress
Detect LIMD1 using this Anti-LIMD1 Antibody, clone 3F2/C6 validated for use in ICC, IHC, Immunoprecipitation, Western Blotting.
Research Category
Apoptosis & Cancer

Stem Cell Research

Evaluated by Western Blot in H1299 cell lysates.
Western Blot Analysis: A 1:2000 dilution of this antibody detected LIMD1 in H1299 cell lysates.

Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Control
H1299 cell lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

3F2/C6, monoclonal

human

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_055055

Q9UGP4

wet ice

~72 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1