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Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12
产品别名
Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12
Electroneutral potassium-chloride cotransporter 2, Erythroid K-Cl cotransporter 2, Neuronal K-Cl cotransporter, electroneural potassium-chloride cotransporter 2, solute carrier family 12, potassium-chloride transporter), member 5
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA′s inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior. |
| Specificity【特异性】 | Shown by an independent laboratory to react in human and mouse tissue. (UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior) This antibody recognizes KCC2 |
| Immunogen【免疫原】 | Recombinant protein corresponding to rat KCC2. Epitope: Unknown |
| Application【应用】 | Detect K+/Cl-Cotransporter (KCC2) using this Anti-K+/Cl-Cotransporter (KCC2) Antibody, clone N1/12 validated for use in IH. Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected KCC2 in rat hypothalamus tissue. Research Category Neuroscience Research Sub Category Ion Channels & Transporters |
| Quality【质量】 | Evaluated by Immunohistochemistry in rat cortex area A-3 tissue. Immunohistochemistry Analysis: 1:300 dilution of this antibody detected KCC2 in rat cortex area A-3 tissue. |
| Physical form【外形】 | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Format: Purified Protein G |
| Analysis Note【分析说明】 | Control Rat cortex area A-3 tissue |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | N1/12, monoclonal |
| species reactivity | rat, human |
| technique(s) | immunohistochemistry: suitable |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NP_599190 |
| UniProt accession no.【UniProt登记号】 | Q63633 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 126 kDa Calculated |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
