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Anti-Hsp47 Mouse mAb (M16.10A1)
产品别名
Anti-Hsp47 Mouse mAb (M16.10A1)
Anti-Colligin
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | M16.10A1, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | bovine, monkey, mouse, human, guinea pig, canine, rat, sheep, porcine, chicken, hamster |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG2b |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein. Recognizes the ~47 kDa Hsp47 protein. Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections). |
Immunogen【免疫原】 | Rat Hsp47 from L6 cells |
Application【应用】 | ELISA (see comments) Enzyme Immunoassay (see comments) Immunoblotting (1 µg/ml, colorimetric) Immunocytochemistry (1 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (5 µg/ml) Paraffin Sections (see comments) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In PBS, 50% glycerol, pH 7.2. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
Analysis Note【分析说明】 | Positive Control 3T3 cells, Human breast cancer tissue |
Other Notes【其他说明】 | Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023. Nandan, D., et al. 1998. Exp. Cell Res.179, 289. Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230. Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793. Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767. Saga, S., et al. 1987. J. Cell Biol.105, 517. Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531. This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |