广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Anti-GGA3 Antibody, clone 3G5.1
产品搜索:

Anti-GGA3 Antibody, clone 3G5.1

产品编号:4154418
规格:clone 3G5.1, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154418100 μG5010
产品别名

Anti-GGA3 Antibody, clone 3G5.1

ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3

基本信息
eCl@ss
32160702
General description【一般描述】
ADP-ribosylation factor-binding protein GGA3 (UniProt: Q9NZ52; also known as Golgi-localized, gamma ear-containing, ARF-binding protein 3) is encoded by the GGA3 (also known as KIAA0154) gene (Gene ID: 23163) in human. GGA3 is a monomeric, ubiquitously expressed protein found in trans-Golgi network (TGN) membrane and endosome membrane. It plays a vital role in protein sorting and trafficking between the trans-Golgi network and endosomes. It mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Four isoforms of GGA3 have been described that are produced by alternative splicing. It contains a VHS domain (aa 16-146) that functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines. Its GAT domain (aa 171-298) is responsible for interaction with ARF-GTP, UBC, and RAB GTPase Binding Effector Protein 1 (RABEP1) and prevents ARF-GTP hydrolysis. GGA3 also has a GAE domain (aa 594-715) that binds accessory proteins regulating GGAs function. GGA3 also contains an auto-inhibitory motif (aa 391-395). GGA3 can be phosphorylated by casein kinase 2 and dephosphorylated by protein phosphatase 2A. GGA3 phosphorylation allows the internal AC-LL motif to bind VHS domain and inhibit the recognition of cargo signals.
Specificity【特异性】
Clone 3G5.1 detects ADP-ribosylation factor-binding protein GGA3 in human and murine cells. It targets an epitope within 103 amino acids from the internal region.
Immunogen【免疫原】
GST/His-tagged recombinant fragment corresponding to 103 amino acids from the internal region of human ADP-ribosylation factor-binding protein GGA3.
Application【应用】
Anti-GGA3, clone 3G5.1, Cat. No. MABN1556, is a highly specific mouse monoclonal antibody that targets ADP-ribosylation factor-binding protein GGA3 and has been tested for use in Western Blotting.
Research Category
Neuroscience
Quality【质量】
Evaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected GGA3 in 10 µg of Neuro-2a cell lysate.
Physical form【外形】
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
3G5.1, monoclonal
species reactivity
mouse, human
packaging【包装】
antibody small pack of 25 μg
technique(s)
western blot: suitable
isotype【同位素/亚型】
IgG2bκ
NCBI accession no.【NCBI登记号】
NP_619525
UniProt accession no.【UniProt登记号】
Q9NZ52
shipped in【运输】
ambient
产品说明
Target description【目标描述】
~80 kDa observed; 78.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0088