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Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | liquid |
does not contain【不包含】 | preservative |
species reactivity (predicted by homology) | mammals |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −70℃ |
packaging【包装】 | 50 μL in Glass bottle |
基本信息
General description【一般描述】 | Recognizes both large and small forms of Gsα-subunit. Does not cross-react with Giα-1-, Giα-2-, Giα-3-, or Goα-subunits. Protein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa Gsα subunit protein. Anti-Gsα-Subunit, C-Terminal (385-394), rabbit polyclonal, recognizes both large and small forms of Gsα-subunit. It is validated for use in Western blotting. |
Immunogen【免疫原】 | a synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH |
Application【应用】 | Immunoblotting (1:1000) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-70°C). |
Analysis Note【分析说明】 | Positive Control Gsα-Subunit, His•Tag |
Other Notes【其他说明】 | Does not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution. Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537. Raymond, J.R., et al. 1993. Biochemistry32, 11064. Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |