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Anti-Fibulin-1 Antibody, clone 3D12.1
产品别名
Anti-Fibulin-1 Antibody, clone 3D12.1
FIBL-1, Fibulin-1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Fibulin-1 (UniProt P23142; also known as FIBL-1) is encoded by the FBLN1 (also known as FIBL1, FBLN, PP213) gene (Gene ID 2192) in human. Fibulin-1 is an extracellular matrix protein that interacts with fibronectin and inhibits cell migration and invasion in a fibronectin-dependent manner. Research shows that the EGF-like domains 5 and 6 of fibulin-1 compete against syndecan-4 for binding the C-terminal heparin-binding domain (HepII) of fibronectin, resulting in a suppression of fibronectin-regulated cell adhesion and motility. Fibulin-1 also interacts with fibrinogen and regulates platelet adhesion to influence hemoglutination. In addition, fibulin-1 interacts with elastin and plays a role in the assembly of elastic fibers by mediating association between versican and fibrillin. Other fibulin-1 interactors include calumenin, extracellular matrix protein 1, angiogenin, and hemicentin. There exist four major fibulin-1 isoforms (A, B, C, and D) generated by alternative splicing, with isoforms C and D being the predominant forms. In addition, a smaller isoform of fibulin-1D, fibulin-1D′, is reported to localize in the cytosol and interacts with the intracellular domain of integrin β1. Fibulin-1 is initially produced with a signal peptide (a.a. 1-29), the removal of which yields the mature protein with three N-terminal Anaphylatoxin-like domains (a.a. 36-76, 77-111, 112-144), followed by eight EGF-like domains (a.a. 176-578 of isoform D), with EGF-like domains 5 & 6 mediating fibulin-1 self-association and fibronectin-binding. The C-terminal 137-amino acid in isoform D is truncated in isoform A, and replaced by a shorter sequence of 35 amino acids in isoform B and a sequence of 117 amino acids in isoform C. |
Specificity【特异性】 | Expected to react with all four spliced isoforms of human fibulin-1 (UniProt P23142-1 to P23142-4). |
Immunogen【免疫原】 | Epitope: EGF-like domains 4-6. GST-tagged recombinant protein corresponding to EGF-like domains 4-6 of human Fibulin-1. |
Application【应用】 | Research Category Cell Structure Detect Fibulin-1 using this Anti-Fibulin-1 Antibody, clone 3D12.1 validated for use in Western Blotting, Immunohistochemistry. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Fibulin-1 in human placenta and human breast tissue. Research Sub Category ECM Proteins |
Quality【质量】 | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: 4.0 µg/mL of this antibody detected Fibulin-1 in 10 µg of SH-SY5Y cell lysate. |
Physical form【外形】 | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 3D12.1, monoclonal |
species reactivity | human |
technique(s) | immunohistochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_001987 |
UniProt accession no.【UniProt登记号】 | P23142 |
shipped in【运输】 | wet ice |
Gene Information | human ... FBLN1(2192) |
产品说明
Target description【目标描述】 | ~77 kDa observed. 77.2 kDa (isoform D), 61.6 kDa (isoform A), 65.4 kDa (isoform B), and 74.4 kDa (Isoform C) calculated. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |