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Anti-E-cadherin Antibody, clone DECMA-1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium-binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas, E-cadherin is frequently downregulated, consistent with its function of an invasion suppressor in normal epithelia. |
Immunogen【免疫原】 | Mouse embryonal carcinoma cell line PCC4 |
Application【应用】 | Research Category Cell Structure This Anti-E-cadherin Antibody, clone DECMA-1 is validated for use in WB, IH, IC, IP, FC, FUNC for the detection of E-cadherin. Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect E-cadherin in mouse GI tract tissue. Inhibition: A previous lot was used by an independent laboratory in inhibion studies, blocking E-cadherin associated cell adhesion. (Vestweber, 1985) Flow Cytometry Analysis: A previous lot was used by an independent laboratory in flow cytometry applications. (Tang, 1994) Research Sub Category Adhesion (CAMs) |
Quality【质量】 | Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate. |
Physical form【外形】 | Purified rat monoclonal IgG1κ in buffer containing PBS without preservative. Format: Purified Protein G |
Analysis Note【分析说明】 | Control A431 cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | Decma-1, monoclonal |
species reactivity | mouse, human |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1κ |
NCBI accession no.【NCBI登记号】 | NP_033994 |
UniProt accession no.【UniProt登记号】 | P09803 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | ~ 106 kDa observed MW. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |