Anti-Bcl-2-associated transcription factor 1 Antibody

32160702

NA.41

Bcl-2-associated transcription factor 1 (Btf) is a ubiquitously expressed death-promoting transcriptional repressor. It has been shown to interact with anti-apoptotic proteins related to Bcl-2, DNA, adenovirus E1B 19K, and EMD. Btf has also been shown to be involved in E1A transformation of p53 or E1B 19K, an interaction that implicates Btf as being involved in transcriptional regulation and in promoting apoptosis. Expression of Btf is absent in some cancers, which may suggest a potential role for the protein in tumor suppression.

Other homologies: Rat (81% sequence homology).

KLH-conjugated linear peptide corresponding to human Bcl-2-associated transcription factor 1.

Western Blot (Snap i.d.) Analysis: 0.5 µg/mL from a previous lot detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Bcl-2-associated transcription factor 1 from HeLa cell lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Bcl-2-associated transcription factor 1 in neuronal and glial cells in normal human brain tissue.
This Anti-Bcl-2-associated transcription factor 1 Antibody is validated for use in WB, IP, IH(P) for the detection of Bcl-2-associated transcription factor 1.
Research Sub Category
BCL2 & Inhibition
Research Category
Apoptosis & Cancer

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, mouse

bovine (based on 100% sequence homology), canine (based on 100% sequence homology)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NP_055554

Q9NYF8

wet ice

~120 kDa observed.
This protein has 4 isoforms: Isoform 1 (106 kDa calculated), Isoform 2 (106 kda calculated), Isoform 3 (100 kDa calculated), Isoform 4 (86 kDa calculated).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1