Anti-Aquaporin 9 Antibody

32160702

NA.41

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane.

A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.

Aquaporin 9. The immunogen peptide is unique to Aquaporin-9 without significant homology to any other Aquaporins.

Rat. The immunogen peptide has 94% conservation with mouse and 72% conservation with the human Aquaporin-9. Species reactivity has not been confirmed.

An 18 AA synthetic peptide within the C-terminal domain of rat Aquaporin-9 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.

Research Category
Neuroscience
Detect Aquaporin 9 using this Anti-Aquaporin 9 Antibody validated for use in ELISA, IC, IH & WB.
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL

ELISA: 1:100,000 using 50-100 ng of control peptide per well.

Immunohisto/cytochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues. Adherent cells can be fixed with 50% methanol-50% acetone or 1% paraformaldehyde.

Optimal working dilutions must be determined by end user.

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

chicken

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

rat

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NM_020980.3

O43315

dry ice

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.

Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2