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Anti-Aquaporin 0 Antibody
产品别名
Anti-Aquaporin 0 Antibody
AQP0, MIP, Major Intrinsic Protein
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. |
Immunogen【免疫原】 | A 17 AA synthetic peptide within the carboxy terminal domain of human Aquaporin-0 (Shiels et al. 1988; Kent et al. 1990; Pisano et al. 1991; Shiels et al. 1996) was selected for antibody production. This domain is predicted to be cytoplasmic. |
Application【应用】 | Detect Aquaporin 0 using this Anti-Aquaporin 0 Antibody validated for use in ELISA & WB. We recommend the use of 0.5-1% milk in all primary/secondary dilutions in order to suppress non-specific bands. Western blot: 1-10 μg/mL using Chemiluminescence technique ELISA: 0.5-1.0 μg/mL Optimal working dilutions must be determined by end user. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_012064.2 |
UniProt accession no.【UniProt登记号】 | P30301 |
shipped in【运输】 | dry ice |
产品说明
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |