Anti-Amyloid Precursor Protein Antibody, KPI Domain

32160702

NA.41

Amyloid Precursor Protein (APP), KPI Domain.

APP.

Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.
Western blot: 1:250 - 1:1,000

Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.

Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.

Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.

Optimal working dilutions must be determined by end user.

Reduction /Alkylation protocol:

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

monkey, mouse, bovine, human

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NM_000484.2
NM_201413.1
NM_201414.1

P05067

dry ice

human ... APP(351)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2